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rabbit anti lc3  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti lc3
    Rabbit Anti Lc3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lc3+antibody/LC3B+Antibody+-+BSA+Free/pm41912478-90-103-106
    Average 96 stars, based on 1663 article reviews
    rabbit anti lc3 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows
    Article Snippet: .. Samples were then blocked in 5% BSA-supplemented PBS for 2 h at room temperature and incubated with rabbit anti-LC3 antibody (NBP1-78964, 1:200 dilution, Novus Biological, Littleton, CO, USA) overnight at 4 °C in a humidified chamber. .. After washing, samples were incubated for 3 h at 25 °C with goat anti-rabbit Alexa Fluor Plus 488-conjugated secondary antibody (A32731, dilution 1:1000, Thermo Fisher Scientific, Shanghai, China) and counterstained with DAPI.

    Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG.
    Article Snippet: Cells were blocked for 1 h in BlockerTM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 ◦C and visualized via incubation with anti-Rabbit IgG Alexa FlourTM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 ◦C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).

    Article Title: The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.
    Article Snippet: .. Samples were then blocked in 5% BSA-supplemented PBS for 2 h at room temperature and incubated with rabbit anti-LC3 antibody (NBP1-78964, 1:200 dilution, Novus Biological, Littleton, CO, USA) overnight at 4 ◦C in a humidified chamber. .. After washing, samples were incubated for 3 h at 25 ◦C with goat anti-rabbit Alexa Fluor Plus 488-conjugated secondary antibody (A32731, dilution 1:1000, Thermo Fisher Scientific, Shanghai, China) and counterstained with DAPI.

    Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG
    Article Snippet: Cells were blocked for 1 h in Blocker TM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 °C and visualized via incubation with anti-Rabbit IgG Alexa Flour TM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 °C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).

    Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
    Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.

    Staining:

    Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG.
    Article Snippet: Cells were blocked for 1 h in BlockerTM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 ◦C and visualized via incubation with anti-Rabbit IgG Alexa FlourTM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 ◦C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).

    Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG
    Article Snippet: Cells were blocked for 1 h in Blocker TM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 °C and visualized via incubation with anti-Rabbit IgG Alexa Flour TM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 °C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).

    other:

    Article Title: Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced tau secretion
    Article Snippet: Rabbit anti-LC3 antibody (1:1000; #NB1002220 Novus Biologicals).

    Sample Prep:

    Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
    Article Snippet: .. Sample preparation for western blot was performed as previously described.60 The primary antibodies used were: mouse anti-a-Syn (1:1000; Insight Biotechnology, Wembley, UK), mouse anti-pSer129 (1:500; Biolegend, San Diego, CA, USA), rabbit anti-S6K1 ribosomal protein (1:500; #2217T, Cell Signaling Technology); rabbit anti-p-S6K1 ribosomal protein (S240/244) (1:500; #2215S, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1000; #NB100-2220, Novus Biologicals); mouse anti-ß-actin (1:1000; A5316, Neuron 113, 1–17.e1–e13, June 18, 2025 e10 Sigma-Aldrich) and mouse-anti-Tubulin (1:5000; #T6199, Sigma-Aldrich). .. Immunoreactive bands were detected by addition of SupersignalWest Pico PLUSChemiluminescent Substrate (Thermo Fisher Scientific) using LI-CORODYSSEY Fc and Image Studio software (version 5.2).

    Western Blot:

    Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
    Article Snippet: .. Sample preparation for western blot was performed as previously described.60 The primary antibodies used were: mouse anti-a-Syn (1:1000; Insight Biotechnology, Wembley, UK), mouse anti-pSer129 (1:500; Biolegend, San Diego, CA, USA), rabbit anti-S6K1 ribosomal protein (1:500; #2217T, Cell Signaling Technology); rabbit anti-p-S6K1 ribosomal protein (S240/244) (1:500; #2215S, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1000; #NB100-2220, Novus Biologicals); mouse anti-ß-actin (1:1000; A5316, Neuron 113, 1–17.e1–e13, June 18, 2025 e10 Sigma-Aldrich) and mouse-anti-Tubulin (1:5000; #T6199, Sigma-Aldrich). .. Immunoreactive bands were detected by addition of SupersignalWest Pico PLUSChemiluminescent Substrate (Thermo Fisher Scientific) using LI-CORODYSSEY Fc and Image Studio software (version 5.2).

    Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
    Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.

    Software:

    Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
    Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.



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    Image Search Results


    LIPUS promotes mitophagy in high glucose-stimulated HUVECs. ( A – D ) Representative protein bands and quantitative analysis of p62, LC3-II, and TOM20 protein levels; one-way ANOVA ( n = 3). ( E ) Immunofluorescence co-staining of LC3 (green), TOM20 (red), and DAPI (blue). The scale bar was set to 20 μm ( n = 3). ( F ) 3D surface plot of LC3 fluorescence. ( G ) Fluorescence intensity distribution profiles for LC3 and TOM20. Green represents LC3; red represents TOM20. ( H ) Quantification of LC3-TOM20 co-localization. Co-localization was quantified with the overlap coefficient calculated with ImageJ (Colocalization Finder plugin). A total of 16–21 cells per group were analyzed; one-way ANOVA ( n = 3); ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent SD of the mean.

    Journal: Metabolites

    Article Title: Regulation of Mitophagy by Low-Intensity Pulsed Ultrasound Attenuates Endothelial Dysfunction

    doi: 10.3390/metabo16050329

    Figure Lengend Snippet: LIPUS promotes mitophagy in high glucose-stimulated HUVECs. ( A – D ) Representative protein bands and quantitative analysis of p62, LC3-II, and TOM20 protein levels; one-way ANOVA ( n = 3). ( E ) Immunofluorescence co-staining of LC3 (green), TOM20 (red), and DAPI (blue). The scale bar was set to 20 μm ( n = 3). ( F ) 3D surface plot of LC3 fluorescence. ( G ) Fluorescence intensity distribution profiles for LC3 and TOM20. Green represents LC3; red represents TOM20. ( H ) Quantification of LC3-TOM20 co-localization. Co-localization was quantified with the overlap coefficient calculated with ImageJ (Colocalization Finder plugin). A total of 16–21 cells per group were analyzed; one-way ANOVA ( n = 3); ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent SD of the mean.

    Article Snippet: The primary antibody, Rabbit anti-LC3 antibody (1:200, Abmart, T55992 ) and mouse anti-TOM20 antibody (1:200, Proteintech, Wuhan, China, 666777-1-Ig), diluted in blocking solution, was applied overnight at 4 °C.

    Techniques: Immunofluorescence, Staining, Fluorescence

    ANXA3 did not regulate myocardial free fatty acid uptake or lipolysis but positively modulated macroautophagy in T2DM mice. (A-C) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of db/m and db/db mice analyzed by Western blot, n = 4. (D-F) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of mice analyzed by Western blot, n = 4. (G-I) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6. (J-L) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6-8. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/m group or the db/db + OE-GFP group; ∗∗ P < 0.01 versus the db/m group or the vehicle group or the OE-NC + vehicle group; ∗∗∗ P < 0.001 versus the vehicle group or the OE-NC + vehicle group; # P < 0.05 versus the OE-NC + HGPA group; ### P < 0.001 versus the OE-NC + HGPA group.

    Journal: Redox Biology

    Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

    doi: 10.1016/j.redox.2026.104085

    Figure Lengend Snippet: ANXA3 did not regulate myocardial free fatty acid uptake or lipolysis but positively modulated macroautophagy in T2DM mice. (A-C) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of db/m and db/db mice analyzed by Western blot, n = 4. (D-F) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of mice analyzed by Western blot, n = 4. (G-I) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6. (J-L) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6-8. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/m group or the db/db + OE-GFP group; ∗∗ P < 0.01 versus the db/m group or the vehicle group or the OE-NC + vehicle group; ∗∗∗ P < 0.001 versus the vehicle group or the OE-NC + vehicle group; # P < 0.05 versus the OE-NC + HGPA group; ### P < 0.001 versus the OE-NC + HGPA group.

    Article Snippet: Blocked with 5% (w/v) non-fat-dried milkat room temperature for 1 h. Then the membranes were incubated with the anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:1000 [v/v]), the anti-YY1 antibody (Proteintech, 22156-1-AP; 1:1000 [v/v]) the anti-PLIN2 antibody (Proteintech, 15294-1-AP; 1:1000 [v/v]), the anti-SQSTM1/p62 antibody (Cell Signaling Technology, 23214; 1:1000 [v/v]), the anti-LC3 antibody (Cell Signaling Technology, 12741; 1:1000 [v/v]), the anti-Rab7 antibody (Proteintech, 55469-1-AP; 1:1000 [v/v])or the rabbit anti-α-Tubulin antibody (ABclonal, AC031; 1:1000 [v/v])overnight at 4 °C.

    Techniques: Expressing, Western Blot