Incubation:Article Title: The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows
Article Snippet: .. Samples were then blocked in 5% BSA-supplemented PBS for 2 h at room temperature and incubated with rabbit anti-LC3 antibody (NBP1-78964, 1:200 dilution, Novus Biological, Littleton, CO, USA) overnight at 4 °C in a humidified chamber. .. After washing, samples were incubated for 3 h at 25 °C with goat anti-rabbit Alexa Fluor Plus 488-conjugated secondary antibody (A32731, dilution 1:1000, Thermo Fisher Scientific, Shanghai, China) and counterstained with DAPI.
Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG.
Article Snippet: Cells were blocked for 1 h in BlockerTM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 ◦C and visualized via incubation with anti-Rabbit IgG Alexa FlourTM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 ◦C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).
Article Title: The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.
Article Snippet: .. Samples were then blocked in 5% BSA-supplemented PBS for 2 h at room temperature and incubated with rabbit anti-LC3 antibody (NBP1-78964, 1:200 dilution, Novus Biological, Littleton, CO, USA) overnight at 4 ◦C in a humidified chamber. .. After washing, samples were incubated for 3 h at 25 ◦C with goat anti-rabbit Alexa Fluor Plus 488-conjugated secondary antibody (A32731, dilution 1:1000, Thermo Fisher Scientific, Shanghai, China) and counterstained with DAPI.
Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG
Article Snippet: Cells were blocked for 1 h in Blocker TM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 °C and visualized via incubation with anti-Rabbit IgG Alexa Flour TM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 °C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).
Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.
Staining:Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG.
Article Snippet: Cells were blocked for 1 h in BlockerTM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 ◦C and visualized via incubation with anti-Rabbit IgG Alexa FlourTM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 ◦C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).
Article Title: Interplay of Impaired Cellular Bioenergetics and Autophagy in PMM2-CDG
Article Snippet: Cells were blocked for 1 h in Blocker TM Casein in PBS (Thermo Fischer). .. Then, cells were stained with rabbit anti-LC3 antibody (Novus Biologicals; Cat# NBP2-46892) overnight at 4 °C and visualized via incubation with anti-Rabbit IgG Alexa Flour TM 488 (Thermo Fischer Scientific; Cat# A-21206) for 1 h at 4 °C. .. These fibroblasts were counterstained with DAPI (Vector Laboratories, Newark, CA, USA; Cat# H-1200).
other:Article Title: Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced tau secretion
Article Snippet: Rabbit anti-LC3 antibody (1:1000; #NB1002220 Novus Biologicals).
Sample Prep:Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
Article Snippet: .. Sample preparation for western blot was performed as previously described.60 The primary antibodies used were: mouse anti-a-Syn (1:1000; Insight Biotechnology, Wembley, UK), mouse anti-pSer129 (1:500; Biolegend, San Diego, CA, USA), rabbit anti-S6K1 ribosomal protein (1:500; #2217T, Cell Signaling Technology); rabbit anti-p-S6K1 ribosomal protein (S240/244) (1:500; #2215S, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1000; #NB100-2220, Novus Biologicals); mouse anti-ß-actin (1:1000; A5316, Neuron 113, 1–17.e1–e13, June 18, 2025 e10 Sigma-Aldrich) and mouse-anti-Tubulin (1:5000; #T6199, Sigma-Aldrich). .. Immunoreactive bands were detected by addition of SupersignalWest Pico PLUSChemiluminescent Substrate (Thermo Fisher Scientific) using LI-CORODYSSEY Fc and Image Studio software (version 5.2).
Western Blot:Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
Article Snippet: .. Sample preparation for western blot was performed as previously described.60 The primary antibodies used were: mouse anti-a-Syn (1:1000; Insight Biotechnology, Wembley, UK), mouse anti-pSer129 (1:500; Biolegend, San Diego, CA, USA), rabbit anti-S6K1 ribosomal protein (1:500; #2217T, Cell Signaling Technology); rabbit anti-p-S6K1 ribosomal protein (S240/244) (1:500; #2215S, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1000; #NB100-2220, Novus Biologicals); mouse anti-ß-actin (1:1000; A5316, Neuron 113, 1–17.e1–e13, June 18, 2025 e10 Sigma-Aldrich) and mouse-anti-Tubulin (1:5000; #T6199, Sigma-Aldrich). .. Immunoreactive bands were detected by addition of SupersignalWest Pico PLUSChemiluminescent Substrate (Thermo Fisher Scientific) using LI-CORODYSSEY Fc and Image Studio software (version 5.2).
Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.
Software:Article Title: Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring.
Article Snippet: .. Membranes were incubated overnight with guinea pig anti-P62 antibody diluted 1/1000 (GP62-C; Progen, Heidelberg, Germany) or rabbit anti-LC3 antibody (NB600-1384; Novus Biologicals, Littleton, CO, USA), followed by an HRPconjugated anti–guinea pig or anti-rabbit antibody (1/5000; Thermo Fisher Scientific), and then visualized by chemiluminescence (Clarity Western ECL Substrate; Bio-Rad, Hercules, CA, USA) using a Gel-Doc imager and Image Lab Software (Bio-Rad). .. The value of P62 and LC3 signal was normalized to the amount of b-actin, revealedwith amouse anti–b actin antibody, (A5441, 1/5000;MilliporeSigma) and expressed as a percentage of the average of controls.
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